Conserved Valproic Acid-Induced Lipid Droplet Formation in Dictyostelium and Human Hepatocytes (huh7) Identifies Structurally Active Compounds
نویسندگان
چکیده
1 Centre for Biomedical Sciences, School of Biological Science, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany Centre for Toxicology, Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, UK
منابع مشابه
Conserved valproic-acid-induced lipid droplet formation in Dictyostelium and human hepatocytes identifies structurally active compounds
Lipid droplet formation and subsequent steatosis (the abnormal retention of lipids within a cell) has been reported to contribute to hepatotoxicity and is an adverse effect of many pharmacological agents including the antiepileptic drug valproic acid (VPA). In this study, we have developed a simple model system (Dictyostelium discoideum) to investigate the effects of VPA and related compounds i...
متن کاملStudy of Valproic Acid-Enhanced Hepatocyte Steatosis
Valproic acid (VPA) is one of the most widely used antiepilepsy drugs. However, several side effects, including weight gain and fatty liver, have been reported in patients following VPA treatment. In this study, we explored the molecular mechanisms of VPA-induced hepatic steatosis using FL83B cell line-based in vitro model. Using fluorescent lipid staining technique, we found that VPA enhanced ...
متن کاملCytoprotective Effects of Hydrophilic and Lipophilic Extracts of Pistacia vera against Oxidative Versus Carbonyl Stress in Rat Hepatocytes
This study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in Pistacia vera againts cytotoxicity, ROS formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). Methanol, water and ethyl acetate were used to ...
متن کاملValproic Acid-Induced Time Dependent Craniofacial Defects in Wistar Rat Fetuse
Purpose: we previously reported that maternal valproic acid (VA) administration during rat pregnancy produced CNS defect ranely, syringomyelia. Furthermore, it seems that administration of valproic acid during critical period of pregnancy may affect on development of other embryonic skeletal portion such as craniofacial region. The goal of our study was to determine whether there is a relations...
متن کاملIron Overload Induced Apoptotic Cell Death in Isolated Rat Hepatocytes Mediated by Reactive Oxygen Species
Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 µM iron resulted in decreased...
متن کامل